For in vitro use only Description: 5x HOT FIREPol® Blend Master Mix is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water). HOT FIREPol® Blend Master Mix contains two carefully optimized enzymes – HOT FIREPol® DNA polymerase and a proofreading polymerase. This enzyme blend has both the 5’→ 3’ exonuclease activity as well as the 3’→ 5’ proofreading activity. HOT FIREPol® Blend Master Mix exhibits an increased fidelity (up to five fold) compared to regular Taq polymerase. In addition, the mix also allows amplification of longer fragments. Generated PCR products are compatible with blunt-end and TA cloning procedures (to increase the blunt end cloning efficiency treat the PCR products with T4 DNA polymerase or DNA polymerase I large Klenow fragment prior to cloning).